Journal: Journal of Clinical Microbiology

Article Title: Leveraging patient data to detect systematic shifts in daptomycin susceptibility testing associated with reduced prescribing

doi: 10.1128/jcm.01510-25

Figure Lengend Snippet: Daptomycin AST results at BJH and HUP. ( A, D ) The monthly fraction of index clinical isolates (e.g., first per patient encounter) of Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, and Staphylococcus epidermidis and testing susceptible (S) or susceptible dose-dependent (SDD). ( B, E ) Daptomycin MIC values for index Enterococcus faecium clinical isolates. ( C, F ) Daptomycin MIC values for the Staphylococcus aureus ATCC 29213 quality control strain. In panel A, the shaded regions indicate the 95% confidence interval. In panels B and C , marker color indicates a result categorized as S or SDD (gray) or not susceptible (red), and lines and shaded regions indicate the monthly median and interquartile range (25th–75th percentile), respectively. In all panels, the vertical dashed lines represent change points determined for the data in the left side of panel A , and the triangle markers denote the timing of institution-specific daptomycin ETEST lot changes. Horizontal lines in C indicate the acceptable CLSI QC range.

Article Snippet: This one doubling dilution shift was mirrored in the QC results at BJH ( ; ) where the median daptomycin MIC for the S. aureus ATCC 29213 QC strain went from 0.5 μg/mL in the baseline epoch to 1.0 ( P < 0.0001) and then back to 0.5 ( P = 0.132) in the affected and recovery epochs, respectively.

Techniques: Control, Marker

Journal: Microbiology Spectrum

Article Title: Phenotypic and genomic characterization of tigecycline heteroresistance in carbapenem-resistant Klebsiella pneumoniae

doi: 10.1128/spectrum.03079-25

Figure Lengend Snippet: Growth curves of KP strains measured at OD 620 (0–12 hours). Parent strains RJ30 and RJ32 and their heteroresistant subclones RJ30_R and RJ32_R were grown in LB; ATCC 700603 was included as a quality-control strain. OD 620 was recorded hourly for 12 hours. Data are mean ± SD; n = 3 technical replicates per strain. Subclones exhibited growth kinetics comparable to their respective parents (2/2 pairs), consistent with μ max and AUC 0–12h analyses (Materials and Methods).

Article Snippet: The QC strain ATCC 700603 exhibited μ max 0.369 ± 0.027 h−1 and AUC 0–12h 4.097 ± 0.128 OD620·h.

Techniques: Control

Journal: Microbiology Spectrum

Article Title: Phenotypic and genomic characterization of tigecycline heteroresistance in carbapenem-resistant Klebsiella pneumoniae

doi: 10.1128/spectrum.03079-25

Figure Lengend Snippet: Time-kill curve of tigecycline heteroresistant strains. Bacterial survival was assessed over 48 hours in the presence of 4× MIC tigecycline. E. coli ATCC 25922 and KP ATCC 700603 served as the control strain.

Article Snippet: The QC strain ATCC 700603 exhibited μ max 0.369 ± 0.027 h−1 and AUC 0–12h 4.097 ± 0.128 OD620·h.

Techniques: Control